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Literature summary for 3.2.2.31 extracted from

  • Wright, P.M.; Yu, J.; Cillo, J.; Lu, A.L.
    The active site of the Escherichia coli MutY DNA adenine glycosylase (1999), J. Biol. Chem., 274, 29011-29018 .
    View publication on PubMed
Search for more literature for 3.2.2.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli PR70/DE3 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
D138N inactive Escherichia coli
K142A the mutant protein is unable to form Schiff base intermediates with DNA substrates. however, the mutant can still bind DNA substrates and has adenine glycosylase activity and promotes a beta/delta-elimination on apurinic DNA Escherichia coli
K157A the mutation has no effect on enzyme activities Escherichia coli
K158A the mutation has no effect on enzyme activities Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O Escherichia coli
-
 ?
-
 ?
DNA containing a cytosine:adenine mismatch + H2O Escherichia coli
-
 ?
-
 ?
DNA containing a guanine:adenine mismatch + H2O Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing a 7,8-dihydro-8-oxo-2'-deoxyguanosine:adenine mismatch + H2O
-
 Escherichia coli ?
-
 ?
DNA containing a cytosine:adenine mismatch + H2O
-
 Escherichia coli ?
-
 ?
DNA containing a guanine:adenine mismatch + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
adenine DNA glycosylase
-
 Escherichia coli
mutY
-
 Escherichia coli